Molecular Markers of Ovarian Germ Cells of Banana Prawn (Fenneropenaeus merguiensis)

The banana prawn (Fenneropenaeus merguiensis) is a valuable in the worldwide market. However, cultivation of this species limited owing to difficulty culture management and knowledge reproduction. Therefore, we studied gene expression molecular mechanisms involved oogenesis for elucidating ovarian germ cell development prawns. tissue-specific distribution certain genes identified from previous transcriptome data showed that FmCyclinB, FmNanos, nuclear autoantigenic sperm protein (FmNASP) were only expressed gonads. situ hybridization (ISH) these three different patterns throughout oogenesis. FmCyclinB was highly pre-vitellogenic oocytes. FmNanos at almost same level during but most late stages. Based on highest mid oocytes, respectively, suggested may suppress before initiation vitellogenesis. Meanwhile, FmNASP detected pre-vitellogenesis. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) analysis supported by ISH based high sub-adult ovaries, which contain In study, found reliable markers prawns also dynamic change mechanism sub-stage We determined levels Our findings provide information further studies reproduction assist their cultivation.